Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Microplate Reader 96 & 384 Thermo Multiskan Ascent w. Bookings are free, but you will need to use iLab to book. SDS-PAGE Assay. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Read the Cuvette 1. Ltd. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. 5 87. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Conclusions We report a peptide-induced super-assembly of enzyme-containing MOFs for programmed enzyme. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. • Tel. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. Softmax Pro GxP Software 6. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The absorbance value of each reaction solution was measured at 405 nm by a Molecular Device SpectraMax M5 microplate reader (Sunnyvale, CA, USA). The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. For a higher . Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModePlate chamber design of the SpectraMax category 1 microplate readers. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax® M4. 00 USD. Synergy H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. 0 out of 5 $ 17,999 $ 16,995. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The drawing shows a standard microplate of 15 mm height covered with a lid. Documentation Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Read one sample or up to 384 inThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). iD3, iD5, i3, i3x, M3, M4, M5, M5e, L, and FlexStation 3 readers. Western blotting assay. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. , Sunny-vale, CA). Microplate reader settings. Before you Begin Materials Required but Not Provided • Fresh, anhydrous. Recertification is recommended every two years. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Small, unbound molecules rotate rapidly in. SpectraMax® M5. Figure 3. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual. The inset, using the same data set, highlights the sensitivity of the assay at low levels of H 2O 2. Qualify the luminescence performance of the SpectraMax . SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. Fill a clean plate with water. Call 516-939-0888 to check. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Fluorescent signals were measured using the SpectraMax/M5 fluorescence spectrophotometer plate reader (Molecular Devices Co. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. Pick the best speed, sensitivity, and price for your needs. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 1. MOLECULAR DEVICES SpectraMax M5 also includes a wide selection of curve-fitting functions, allowing users to perform accurate and reliable quantification of biochemical reactions. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. HTRF-compatible SpectraMax Multi-Mode Readers. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. The PBS solution (10. M3 M4 M5 M5e LM1 v<nnn>. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Spectramax M5 Microplate Reader, supplied by Danaher Inc, used in various techniques. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Italic and boldface type are used for emphasis. When the user applies a set temperature of 37°C, the top is heated to 37. 6-384 (96- and 384-well with pipetting) 6-384. The absorbance was measured at 490 nm using a SpectraMax® M5 microplate reader (Molecular Devices, USA). Design of genetic materials Sequence of β-gal siRNA was designed (5'-CA GUU GCG CAG CCU GAA UGU U3', 5'AA CAU UCA GGC UGC GCA ACU G-3') and nonsense siRNA ( Silencer ® negative control siRNA). Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Make sure the microplate is flat against. 00. 04 to 3. for ValitaTiter Plus standards read on Molecular Devices readers. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. 7 cells and HEK293T cells were seeded at the density of 2 × 105 cells per well in a 24-well plate overnight. 7 cells were pretreated with SG (0, 25, 50, and 100 μg/mL). IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175)The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Absorbance was read on a Spectramax M5 Microplate reader at 450 nm. 1** Version 7. Five. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. spectramax m5 multi. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplateinstalled if you are using a SpectraMax ABS microplate reader **SoftMax Pro Software version 7. analysis, controls the SpectraMax Multi-Mode Microplate Readers. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 05 3. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. For more sample throughput, the system can Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. 2. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Description. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. M3-M5e_manual. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. (Shanghai, China). The assay window spanned four orders of magnitude. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. AdThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Or scan up to 384 wells at any time to determine the best assay conditions. Then, 100 μL MTT lysis buffer was added to each well and incubated for 16 h. System Diagrams and Dimensions. 6-384. Expanded wavelength coverage. temperature and fluorescence was measured at Ex/Em 490/585 nm using a Molecular Devices SpectraMax M5 microplate reader. 3. The SpectraMax M5e Multi. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. 314 low lamp level warning. The K d was calculated with GraphPad Prism software (GraphPad Software Inc. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. Western blot analyses. The percentage of viable cells for each treatment group was calculated by adjusting the untreated control group to 100%. 6: bioNova científica, s. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. Figure 1. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Category 2 and 3 microplate readers use gradient heating as well. Detailed specifications of each model reader are reported in Section 7, “Specifications”. M3-M5e_manual. Molecular Devices SpectraMax M5 ; Molecular Devices SpectraMax M2. TR-FRET assay principle. 67. Then, 150 μL of dimethyl sulfoxide was added to each well and shaken gently for 10 min. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Microplate Readers. 5–100mg/L (ValitaTiter) or 100–2000mg/L (ValitaTiter Plus). 95. Measurements of fluorescence and fluorescence polarization were performed on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA) with the excitation and emission wavelengths 544 nm and 590 nm, respectively. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precision SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Plate chamber design of the SpectraMax category 1 microplate readers. No filter change with dual monochromator optics. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Plates were then shaken at room temperature for 10 min and the absorbance at 570/630 nm was measured by using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. SpectraMax 250 User. The Spectr. Published Dec 16, 2020 | Updated Dec 16, 2020. The dual monochromator optics allow the widest range of applications to be utilized for bior. Examples: “Press carefully to engage,” “ Do not. S. No additional manuals are available. Note: In this user guide, SpectraMax ® refers to several SpectraMax . book Page 7 Wednesday, July 7, 2010 2:09 PM. Flexible for 6-well to 384-well microplates and cuvettes. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. These lab workhorses are used in drug discovery, research, bioassay validation, and. When a FlexStation® 3 Multi-Mode Microplate Reader. E Models Only) 69. Linear, Orbital Slow, and Orbital Fast. SpectraMax M5 Multi-Mode Microplate Reader. The TR-FRET signal was read on a TR-FRET microplate reader (SYNERGY H1, Biotek, Inc). Parallel negative control assays had primary, secondary, or both antibodies omitted. This application note discusses the principles on which PathCheck iswells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Molecular Devices Spectramax M5 Microplate Reader; Related products. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. 49 2. When a feature or capability applies to only certain readers, this exception is noted. 7 1. Ltd. To block unwanted residual excitation light and minimize background interference, a 570 nm. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Figure 1. 0. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. The CellTiter-Glo assay from Promega uses luciferase. A: Microplate Preparation. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. The spectrophotometric. E Models. Microplate Readers are modular and . 1. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Both readers feature an 8-channel read head for fast reads and. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. SpectraMax® ABS Plus. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The cells were incubated with compounds at the indicated doses for 1 h. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. 303 unable to cal dark current. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Western blotting assays were conducted according to previous studies . SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E. The raw data from each individual experiment were normalized to vehicle-treated HK-2. The dual monochromator optics allow. 00 USD. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. 1. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. microplate reader. When a feature or capability applies to only certain readers, this exception is noted. When a feature or capability applies to only certain readers, this exception is noted. Table of Contents. Molecular Devices Spectramax M5 Microplate Reader. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. The SpectraMax M2 and M2e are versatile and reliable. All assays were performed in at least three individual experiments, each comprising no. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Microplate Readers include the M3, M4, M5, and M5e models. Bottom-reading is usually recommended for cell-based assay with adherent cells. 2. This assay is based on the disappearance of hydrogen peroxide (H 2 O 2) at 240 nm in a medium containing 20 mM H 2 O 2, 0. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. Table of Contents. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Compatible automation solutions for SpectraMax Readers. 2. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Microplate Readers include the M3, M4, M5, and M5e models. SpectraMax® M5e. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. The CDWIA was performed on each. Molecular Devices SpectraMax® series microplate readers. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. STAKMAX. p. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. It measures the optical density (OD) of samples in a microplate or in a cuvette at aThe cells were then washed three times with PBS, and the fluorescent intensity of the cells was measured with a SpectraMax M5 microplate reader (Molecular Devices, USA) at an excitation wavelength of 488 nm and emission wavelength of 525 nm. Molecular Devices. com. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. 7 cells (6 × 10 5 cells/well in 6-well plates) were pretreated with of 3,3′,4,5′-TMS or 3,4′,5-TMS at 10 and 50 µM for 4 h, followed by the addition of LPS (1 µg/mL) for 12 h. FlexStation™ I. The SpectraMax M2 and M2e are versatile and reliable. When a The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader;. Microplate Capacity. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Microbiota DNA was. Figure 1. The dual monochromator optics allow the widest range of applications to be. It cannot be used for luminescence measurements. The following document is intended to demonstrate setup of this instrument. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Equal amount of proteins were loaded and resolved using denaturing gel and analyzed by western blot using antibodies recognizing Akt, rS6, mOTR and. Methods Preparation of reagents and standards. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. es •. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). view more . The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. The following document is intended to demonstrate setup of this instrument. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. 05% Tween 20 using a Nunc ELISA plate washer. Five preferred detection modes. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Download PDF. 1 second. 6 for further experiment, so I'm confused which i would choose for OD value determination. Equipped with scanning, shaking and kinetics modules. AquaMax® 2000. This reader's dual monochromators for. The detection of lactate dehydrogenase releaseBriefly, algae were harvested, centrifuged, and re-suspended with an equal volume of methanol at the end of the experiment. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. 0 μL) instead of the sample solution was applied as control group, and. Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. A serial dilution of IgG standards was performed, using XP Media/L-glutamine as the diluent, to concentrations from 2. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. The absorbance was measured at 570 nm using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). 6 0. SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. 96-1536. Fresh cecum content samples were collected for 16S rRNA sequencing by Shanghai Majorbio Bio-Pharm Technology Co. Key applications: ELISA, nucleic acid, endotoxin testing. Molecular Devices. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. When a feature or capability applies to only certain readers, this exception is noted. Turn on the microplate reader. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. 2. metrically at 540 nm using a microplate reader (SpectraMax 384 Plus, Molecular Devices Corp. Cell viability was assayed by addition of BacTiter-Glo ™ bioluminescence reagent according to the manufacturer’s protocol (Promega) and luminescence measured using a Spectramax M5 microplate reader (Molecular Devices). SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. SpectraMax® M5e. Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. All reagents were brought to room temperature before use. 2. both cuvette-based and microplate reader applications on the same instrument. Fluorescence Microplate Reader Comparison Chart. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. For the Live/Dead assay, they were treated. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Fluorescence Microplate Readers. SpectraMax M Series multi-mode microplates are configurable readers with triple. Molecular Devices Spectramax M5e Multi Mode Microplate Reader; Related products. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. 67. 26 1. For readers without a cuvette port, these options are not shown in the software. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Standard SDS-PAGE was performed with a 10% (w/v) separating gel and a 4% stacking gel. ,. SpectraMax® ABS Plus. Cables. Figure 1. 59 6. The SoftMax® Touch. Path Check Pathlength Measurement SpectraMax Spectrofluorometers. When wells are assigned to template groups, data will populate group tables where analysis canThe SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Choose from a three- (M3), four- (M4), or The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT.